Probing mechanotransduction in living cells by optical tweezers and FRET-based molecular force microscopy
نویسندگان
چکیده
منابع مشابه
Imaging molecular interactions in living cells by FRET microscopy.
Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function. The complex underlying network of interactions reflects elementary reactions operating under s...
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Mechanical forces affect biological systems in their natural environment in a widespread manner. Mechanical stress may either stimulate cells or even induce pathological processes. Cells sensing mechanical stress usually respond to such stressors with proliferation or differentiation. Hence, for in vitro studies, the ability to impose a controlled mechanical stress on cells combined with approp...
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Atomic force microscopy (AFM) allows the acquisition of high-resolution images and the measurement of mechanical properties of living cells under physiological conditions. AFM cantilevers with blunted pyramidal tips are commonly used to obtain images of living cells. Measurement of mechanical properties with these tips requires a contact model that takes into account their blunted geometry. The...
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ژورنال
عنوان ژورنال: The European Physical Journal Plus
سال: 2021
ISSN: 2190-5444
DOI: 10.1140/epjp/s13360-021-01273-7